| 质粒名称: | pULTRA-CNF |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 48215 |
| 存储实验室: | Peter Schultz |
|
载体骨架基本信息 |
|
| 载体名称: | pULTRA |
| 载体出品公司: | Schultz lab |
| 完整质粒大小: | 4977bp |
| 载体类型: | 细菌细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称1: | polyspecific MJ tyrosyl synthetase |
| 物种来源: | M. jannaschii |
| 基因突变: | TY32L, L65V, F108W, Q109M, D158G, I159A |
| 启动子: | tacI |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NotI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物： | Tac promoter |
| 插入基因名称2: | tRNA |
| 启动子: | proK |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SbfI (unknown if destroyed) |
| 3’克隆位点: | NcoI (unknown if destroyed) |
| 5’测序引物： | na |
|
文献引用方法 |
|
| 材料和方法部分: | pULTRA-CNF was a gift from Peter Schultz (Addgene plasmid # 48215 ; http://n2t.net/addgene:48215 ; RRID:Addgene_48215) |
| 参考文献部分: | A genetically encoded infrared probe. Schultz KC, Supekova L, Ryu Y, Xie J, Perera R, Schultz PG. J Am Chem Soc. 2006 Nov 1;128(43):13984-5. 10.1021/ja0636690 PubMed 17061854 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pULTRA-CNF 4977 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION highly efficient suppressor plasmid.
ACCESSION .
VERSION .
KEYWORDS pULTRA-CNF
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4977)
AUTHORS Schultz KC, Supekova L, Ryu Y, Xie J, Perera R, Schultz PG
TITLE A genetically encoded infrared probe.
JOURNAL J Am Chem Soc. 2006 Nov 1;128(43):13984-5.
PUBMED 17061854
REFERENCE 2 (bases 1 to 4977)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4977
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 11..39
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 47..63
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind complement(1068..1085)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
regulatory 1709..1718
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS complement(1901..2692)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
promoter complement(2693..2784)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2832..3570)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
CDS complement(3780..4862)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 4824..4843
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(4863..4940)
/gene="lacI"
/label=lacI promoter
ORIGIN
1 tagggagctg ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa
61 caatttcaca aaggaggtgc ggccgcatgg acgaatttga aatgataaag agaaacacat
121 ctgaaattat cagcgaggaa gagttaagag aggttttaaa aaaagatgaa aaatctgctc
181 tgataggttt tgaaccaagt ggtaaaatac atttagggca ttatctccaa ataaaaaaga
241 tgattgattt acaaaatgct ggatttgata taattatagt tttggctgat ttacatgcct
301 atttaaacca gaaaggagag ttggatgaga ttagaaaaat aggagattat aacaaaaaag
361 tttttgaagc aatggggtta aaggcaaaat atgtttatgg aagtgaatgg atgcttgata
421 aggattatac actgaatgtc tatagattgg ctttaaaaac taccttaaaa agagcaagaa
481 ggagtatgga acttatagca agagaggatg aaaatccaaa ggttgctgaa gttatctatc
541 caataatgca ggttaatggt gctcattatc ttggcgttga tgttgcagtt ggggggatgg
601 agcagagaaa aatacacatg ttagcaaggg agcttttacc aaaaaaggtt gtttgtattc
661 acaaccctgt cttaacgggt ttggatggag aaggaaagat gagttcttca aaagggaatt
721 ttatagctgt tgatgactct ccagaagaga ttagggctaa gataaagaaa gcatactgcc
781 cagctggagt tgttgaagga aatccaataa tggagatagc taaatacttc cttgaatatc
841 ctttaaccat aaaaaggcca gaaaaatttg gtggagattt gacagttaat agctatgagg
901 agttagagag tttatttaaa aataaggaat tgcatccaat ggatttaaaa aatgctgtag
961 ctgaagaact tataaagatt ttagagccaa ttagaaagag attataagcg gccgcgttta
1021 aacggtctcc agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt
1081 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
1141 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
1201 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
1261 gaaagactgg gccttgtttg tgagctcccg gtcatcaatc atccccataa tccttgttag
1321 cctgcaggta attccgcttc gcaacatgtg agcaccggtt tattgactac cggaagcagt
1381 gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta
1441 ataattgacg atatgatcag tgcacggcta actaagcggc ctgctgactt tctcgccgat
1501 caaaaggcat tttgctatta agggattgac gagggcgtat ctgcgcagta agatgcgccc
1561 cgcattccgg cggtagttca gcagggcaga acggcggact ctaaatccgc atggcagggg
1621 ttcaaatccc ctccgccgga ccaaattcga aaagcctgct caacgagcag gcttttttgc
1681 atgctcgagc agctcagggt cgaatttgcc atggcggcca ccaggtacca ccggcgcctc
1741 aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc
1801 aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg tcatcgtggc
1861 cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg
1921 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
1981 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
2041 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
2101 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
2161 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
2221 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
2281 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
2341 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
2401 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
2461 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
2521 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
2581 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
2641 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atactcttcc
2701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
2761 aatgtattta gaaaaataaa caaatagcta gctcactcgg tcgctacgct ccgggcgtga
2821 gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg
2881 ggactaacat gtgaggcaaa acagcagggc cgcgccggtg gcgtttttcc ataggctccg
2941 ccctcctgcc agagttcaca taaacagacg cttttccggt gcatctgtgg gagccgtgag
3001 gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt
3061 ttccggcggg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc
3121 tgttccgcct ttctccctta cgggaagtgt ggcgctttct catagctcac acactggtat
3181 ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag
3241 cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccgga aaagcacggt
3301 aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa
3361 acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg
3421 ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct
3481 tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga
3541 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgctcta gatttcagtg
3601 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc
3661 tcatgttagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
3721 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct
3781 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
3841 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
3901 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
3961 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
4021 catgagctgt cttcggtatc gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc
4081 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
4141 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
4201 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
4261 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
4321 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag
4381 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
4441 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
4501 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
4561 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
4621 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
4681 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
4741 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
4801 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
4861 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
4921 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcat
//
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